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Image Search Results
Journal: PLoS ONE
Article Title: Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm
doi: 10.1371/journal.pone.0191570
Figure Lengend Snippet: (A) Illustration of sample annotation and basic profile filter parameters. Shading indicates that additional replicates are optional. FC = fold change. (B) Screen-shot from the ProFED online tool showing the “slider” panel used to set fold change thresholds and limits of desired hit profiles. The depicted setting filters for targets specific to condition a . (C) Exemplary read count profiles of hits specific to condition a (A673; top panel) and b (HEK293; bottom panel) as generated by ProFED. (D) STRING analysis of reported and predicted protein-protein interactions among condition a (A673)-specific hits. 13 hits filtered with a stringent ProFED profile were analyzed in comparison to 28 hits based on a more open hit profile ( plus ). Line thickness indicates the strength of data support.
Article Snippet: To filter shRNAs for hits according to fold change profiles, we created the
Techniques: Generated, Protein-Protein interactions, Comparison
Journal: PLoS ONE
Article Title: Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm
doi: 10.1371/journal.pone.0191570
Figure Lengend Snippet: Screen hits specific to the A673 cell line (condition a ) as filtered using ProFED.
Article Snippet: To filter shRNAs for hits according to fold change profiles, we created the
Techniques: Binding Assay
Journal: PLoS ONE
Article Title: Target discovery screens using pooled shRNA libraries and next-generation sequencing: A model workflow and analytical algorithm
doi: 10.1371/journal.pone.0191570
Figure Lengend Snippet: Enriched gene ontologies of A673 (condition a )-specific hits.
Article Snippet: To filter shRNAs for hits according to fold change profiles, we created the
Techniques: Phospho-proteomics, Transduction